目的:
Autophagy, as a process of degrading damaged proteins and organelles in cells, has become a new approach to explore drug resistance in cancer cells. As an intravesical instillation of doxorubicin, can doxorubicin induce autophagy, which is a positive feedback, promoting cell death, or a negative feedback that inhibits cell death? It's not clear yet. The purpose of this study is to investigate the role of autophagy in adriamycin resistant bladder cancer. 

方法: the expression of autophagy related proteins by Western blot assay of pirarubicin in the role of EJ and J82 in bladder cancer cells after; the autophagy inhibitor 3- methyl adenylate, hydroxychloroquine, siRNA regulates autophagy flux, further confirmation of pirarubicin induced bladder cancer cell autophagy.

结果: the expression levels of autophagy related proteins ATG3 and LC3 increased gradually with the increase of adriamycin concentration and the prolongation of the action time, and showed a concentration dependent and time-dependent manner. For EJ and J82 in bladder cancer cell autophagy inhibitor 3- early after treatment with ATG3 or methyl adenylate silence pirarubicin induced autophagy, autophagy is inhibited; give advanced inhibitor hydroxychloroquine cells treated with pirarubicin induced LC3 accumulation is more obvious.

结论: adriamycin can modulate autophagy flux in bladder cancer cells and induce autophagy.