您的位置: 首页 >会议日程
施少林
单位: 中国人民解放军东部战区总医院 科室: 肾内科
简历:

                             施少林简历                                       
学历:博士
专业技术职称:主任技师
主要研究方向:肾小球疾病的发病机制和足细胞生物学 
工作单位:解放军南京总医院国家肾脏病临床研究中心
地址:江苏省南京市中山东路305号实验楼508室
电话:13675109012
邮箱:shaolinshi1001@yahoo.com
教育经历
1982 – 1986 厦门大学生物学专业,本科生
1989 – 1992 复旦大学基因工程与遗传学专业,硕士生
1994 – 1997 复旦大学遗传学研究所,博士生
工作经历
1986 – 1989      江西中医学院基础医学部,助教
1992 – 1994      深圳晶美生物技术有限公司,研究助理
1997 – 1998      英国Glasgow大学/Cyclacel制药有限公司,博士后
1998 – 2004      美国爱因斯坦医学院(Albert Einstein)细胞生物学系,博士后 
2005 – 2011     美国纽约西奈山医学院(Ichan School of Medicine at Mount Sinai)医学部肾脏科,助理教授
2011 –            解放军南京总医院国家肾脏病临床研究中心,主任技师
奖 励
- 2016年江苏省科技进步一等奖:“足细胞损伤机制和临床转化研究”(排名第四);
- 2011年江苏省“双创”人才计划
杂志编委
- Frontiers in Renal and Epithelial Physiology
- Kidney Diseases
科研基金
课题负责人基金
国家自然科学基金 面上项目(81670653):“新的足细胞损伤基因PAWR的鉴定及其作用机制”,2017-2020
国家自然科学基金 面上项目(81370827):“循环线粒体DNA在足细胞损伤中的作用及其机制”,2014-2017
江苏省自然科学基金面上项目(BK20131324):“线粒体DNA激活的TLR9信号通路在肾小球足细胞损伤中的作用及其机制”,2014-2016
江苏省临床研究中心糖尿病肾病子课题(BL2012007):2012-2015
江苏省双创人才计划(2011):2012-2015
国家自然科学基金 青年基金(39700027):“β-半乳糖苷酶样基因的克隆和表征”,金额: 1997 - 2000
 
重大课题研究骨干和主要参与人
国家973重大基础研究计划(2012CB517600):“肾小球疾病的分子机制和生物标记物研 究”,2012 – 2016
江苏省自然科学基金重点项目(BK2011020): “肾脏病生物标本库的构建”,2012- 2016

发表论著:
SCI期刊
1. Rang Y, Wu J, Zheng C, Zhao Y, Zhang S, Wang X, Lu Y, Shi S*, Liu Z*. MiR-30 Family Prevents uPAR-ITGB3 Signaling Activation through Calcineurin-NFATC pathway to protect podocytes. J Biol Chem. 2018 (Revision) (*通讯作者)
2. Yang Q, Sun M, Chen Y, Lu Y, Ye Y, Song H, Xu X, Shi S*, Wang J*. Triptolide protects podocytes from TGF-β-induced injury by preventing miR-30 downregulation. Am J Transl Res. 2017; 9(11): 5150-5159.  (*通讯作者)
3. Lu Y, Ye Y, Bao W, Yang Q, Wang J, Liu Z, Shi S*. Genome-wide identification of genes essential for podocyte cytoskeletons based on single-cell RNA sequencing. Kidney Int. 2017v; 92(5): 1119- 1129.  (*通讯作者)
4. Lu Y, Ye Y, Yang Q, Shi S*. Single-cell RNA-sequence analysis of mouse glomerular mesangial cells uncovers mesangial cell essential genes. Kidney Int. 2017;92(2):504-513.(*通讯作者)
5. Qi H, Casalena G, Shi S, Yu L, Ebefors K, Sun Y, Zhang W, D'Agati V, Schlondorff D, Haraldsson B, Böttinger E, Daehn I. Glomerular Endothelial Mitochondrial Dysfunction Is Essential and Characteristic of Diabetic Kidney Disease Susceptibility. Diabetes. 2017; 66(3):763-778. 
6. Zhao Y, Wu J, Zhang M, Zhou M, Xu F, Zhu X, Zhou X, Lang Y, Yang F, Shi S*, Liu Z*. Angiotensin II Induces Calcium/Calcineurin Signaling and Podocyte Injury by Downregulating MicroRNA-30 Family Members. J Mol Med, 2017;95(8):887-898.  (*通讯作者)
7. Bao W, Xia H, Liang Y, Ye Y, Lu Y, Xu X, Duan A, He J, Chen Z, Wu Y, Wang X, Zheng C, Liu Z, Shi S*. Toll-like Receptor 9 Can be Activated by Endogenous Mitochondrial DNA to Induce Podocyte Apoptosis. Sci Rep. 2016, 6:22579.  (*通讯作者)
8. Wu J, Zheng C, Wang X, Yun S, Zhao Y, Liu L, Lu Y, Ye Y, Zhu X, Zhang C, Shi S*, Liu Z*. MicroRNA-30 family members regulate calcium/calcineurin signaling in podocytes. J Clin Invest. 2015, 125:4091-106.  (*通讯作者)
9. Chen Z, Wan X, Hou Q, Shi S, Wang L, Chen P, Zhu X, Zeng C, Qin W, Zhou W, Liu Z. GADD45B mediates podocyte injury in zebrafish by activating the ROS-GADD45B-p38 pathway. Cell Death Dis. 2016, 7:e2068.
10. He J, Lu Y, Xia H, Liang Y, Wang X, Bao W, Yun S, Ye Y, Zheng C, Liu Z*, Shi S*. Circulating Mitochondrial DAMPs Are Not Effective Inducers of Proteinuria and Kidney Injury in Rodents. PLoS One. 2015, 10:e0124469.  (*通讯作者)
11. Zhang C, Zhang W, Chen HM, Liu C, Wu J, Shi S*, Liu ZH*. Plasma microRNA-186 and proteinuria in focal segmental glomerulosclerosis. Am J Kidney Dis. 2015; 65(2):223-32. (*通讯作者)
12. Zhang W, Zhang C, Chen H, Li L, Tu Y, Liu C, Shi S, Zen K, Liu Z. Evaluation of microRNAs miR-196a, miR-30a-5P, and miR-490 as biomarkers of disease activity among patients with FSGS. Clin J Am Soc Nephrol. 2014; 9(9):1545-52. 
13. Zeng C, Fan Y, Wu J, Shi S, Chen Z, Zhong Y, Zhang C, Zen K, Liu Z. Podocyte autophagic activity plays a protective role in renal injury and delays the progression of podocytopathies. J Pathol. 2014; 234(2):203-13. 
14. Casalena G, Krick S, Daehn I, Yu L, Ju W, Shi S, Tsai SY, D'Agati V, Lindenmeyer M, Cohen CD, Schlondorff D, Bottinger EP. Mpv17 in mitochondria protects podocytes against mitochondrial dysfunction and apoptosis in vivo and in vitro. Am J Physiol Renal Physiol. 2014; 306(11):F1372-80. 
15. Daehn I, Casalena G, Zhang T, Shi S, Fenninger F, Barasch N, Yu L, D'Agati V, Schlondorff D, Kriz W, Haraldsson B, Bottinger EP. Endothelial mitochondrial oxidative stress determines podocyte depletion in segmental glomerulosclerosis. J Clin Invest. 2014; 124(4):1608-21. 
16. Bao H, Hu S, Zhang C, Shi S, Qin W, Zeng C, Zen K, Liu Z. Inhibition of miRNA-21 prevents fibrogenic activation in podocytes and tubular cells in IgA nephropathy. Biochem Biophys Res Commun. 2014; 444(4): 455-60. 
17. Shi S*, Yu L, Zhang T, Qi H, Xavier S, Ju W, Bottinger E*. Smad2-dependent downregulation of miR-30 is required for TGF-β-induced apoptosis in podocytes. PLoS One. 2013; 8(9):e75572.  (*通讯作者)
18. Liu J, Chen Z, Zhang Y, Zhang M, Zhu X, Fan Y, Shi S, Zen K, Liu Z. Rhein protects pancreatic β-cells from dynamin-related protein-1-mediated mitochondrial fission and cell apoptosis under hyperglycemia. Diabetes. 2013; 62(11):3927-35.
19. Gentle ME*, Shi S*, Daehn I, Zhang T, Qi H, Yu L, D'Agati VD, Schlondorff DO, Bottinger EP. Epithelial cell TGFβ signaling induces acute tubular injury and interstitial inflammation. J Am Soc Nephrol. 2013; 24(5):787-99.  (*共同第一)
20.Lu L, Hou X, Shi S, Korner C, and Stanley P.  Slc35c2 promotes Notch1 fucosylation and is required for optimal Notch signaling in mammalian cells. J Biol Chem. 2010; 285:36245-54. 
21. Shi S,* Yu L, Chiu C, Sun Y, Chen J, Khitrov G, Merchenschlager M, Zhang W, Mundel P, and Bottinger E*. Podocyte-selective deletion of Dicer induces proteinuria and glomerulosclerosis.  J Am Soc Nephrol. 2008; 19:2159-2169.  (*通讯作者)
22. Kim M, Chandrasekharan K, Glass M, Shi S, Stahl M, Kaspar B, Stanley P, Martin PT. O-fucosylation of muscle Agrin determines its ability to cluster Acetylcholine receptors. Mol Cell Neurosci. 2008; 39: 452-464, 
23. Krick S, Shi S, Ju W, Faul C, Mundel P, Bottinger E. Mpv17l protects against mitochondrial oxidative stress and apoptosis by activation of Omi/HtrA2 protease.. Proc Natl Acad Sci. 2008; 105: 14106-11. 
24. Shi S, Ge C, Luo Y, Haltiwanger R, Stanley P. The Threonine that carries fucose, but not fucose, is required for Cripto to facilitate Nodal signaling. J Biol Chem. 2007; 282(28):20133-41. 
25. Stahl M, Ge C, Shi S, Pestell R, Stanley P. Notch1-induced transformation of RKE-1 cells requires upregulation of Cyclin D1. Cancer Res. 2006; 66(15): 7562-7570. 
26. Buono K, Robinson G, Martin C, Shi S, Stanley P, Tanigaki K, Honjo T and Hennighausen L. The canonical Notch/RBP-J signaling pathway controls the balance of cell lineages in mammary epithelium during pregnancy. Dev Biol. 2006; 293(2):565-580. 
27. Chen J, Lu L, Shi S, Stanley P. Expression of Notch signaling pathway genes in mouse embryos lacking beta4-galactosyltransferase-1. Gene Expression Pattern. 2006; 6(4):376-382. 
28. Shi S, Stahl M, Lu L, Stanley P. Canonical Notch Signaling is dispensable for early cell fate specifications in mammals. Mol Cell Biol. 2005; 25(21): 9503-9508.   
29. Stanley P, Sundaram S, Tang J and Shi S. Molocular analysis of three gain-of-function CHO mutants that add the bisecting GlcNAc to N-Glycans. Glycobiology. 2005; 15(1):43-53. 
30. Shi S, Williams S, Seppo A, Chen W, Kurniawan H, Ye Z, Marth J and Stanley P. Inactivation of the Mgat1 gene in oocytes impairs oogenesis but embryos lacking complex and hybrid N-glycans develop and implant. Mol Cell Biol, 24(22):9920-9929, 2004. 
31. Shi S and Stanley P. Protein O-fucosyltransferase 1 is an essential component of Notch signaling pathways.  Proc Natl Acad Sci U S A, 100(9):5234-5239, 2003.  
32. Wang Y, Shao L., Shi S, Harris R, Spellman M, Stanley P, and Haltiwanger R. Modification of epidermal growth factors with O-Fucose: cloning and expression of the GDP-Fucose:polypeptide O-Fucosyltransferase.  J  Biol Chem, 276 (43):40338-30345, 2001. 
33. Patnaik S, Zhang A, Shi S, and Stanley P. alpha(1,3) fucosyltransferase expressed by the gain-of-function Chinese hamster ovary glycosylation mutants LEC12, LEC29 and LEC30. Arch Biochem Biophys 375(2):322-332, 2000.
34. Han Z, Zhang Q, Ye M, Kan L, Gu B, Shi S, Zhou J, Fu G, Mao, M, Chen S, Long Y, Chen Z. Molecular cloning of six novel Kruppel-like zinc finger genes from hematopoietic cells and identification of a novel transregulatory domain KRNB.   J Biol Chem, 274(50):35741-35748, 1999. 
35. Liu Y, Yu L, Yu W, Shi S, Sun B, Wu G, and Huang Z. Expression and purification of a new zinc finger gene - ZNF191 protein and its zinc finger region ZNF191(243-368). Sci China (series B), 42(3):245-252, 1999.
36. Shi S, Yu L, Wu G, Liu M, Zheng Q, Fan Y, Zhao S and Tan J. Cloning of the complete cDNA of ZNF191, a novel human zinc finger protein gene.  Prog Nat Sci, 8(4):502 -505, 1998. 
37. Fan Y, Yang R, Yu L, Wu M, Shi S, Ren M, Han Y, Hu J, Zhao S. Identification of a novel missense mutation in Wilson's disease gene. Chin Med J, 1997, 110:887-890.
国内期刊
1. 何静,夏虹,张昌明,刘志红,施少林*。线粒体DNA拷贝数检测方法的建立及在肾脏疾病诊断中的应用. 肾脏病与透析肾移植杂志,23:342-348,2014。(*通讯作者).
2. 夏虹,梁耀军,何静,鲍文朵娜,周显光,张明超,刘志红,施少林*。Toll样受体9在嘌呤霉素氨基核苷导致足细胞损伤中的作用。肾脏病与透析肾移植杂志,23(4),207-215,2014.  (* 通讯作者
3. 张婉芬,陈慧梅,张昌明,施少林,刘志红。尿液miR-30a-5p 预测激素治疗局灶节段性肾小球硬化的疗效。 肾脏病与透析肾移植杂志, 2014, 23(6):517-522.
4. 任兆瑞,施少林,蒋晓兵,严维耀,雷呈祥。2 个棒状杆菌质粒pXZ10142 和pXZ10145. 1 及其转座子TN45 的特征分析。复旦学报(自然科学版),43(2):200-205, 2004.
5. 毕安定,王顺德,王小柯,余龙,施少林,张琪,赵寿元。同源模建法建立和检验人类新基因ZNF191 锌指基序的三维构象. 复旦学报(自然科学版), 37(2): 123-128, 1998.
6. 施少林,刘孟珉,余龙,陈赛娟,郑其平,吴国俊,陈竺, 赵寿元。用人/啮齿类体细胞杂种系及荧光原位杂交将人类新基因ZNF191 定位于染色体18q12. 1。实验生物学报,  31(1):21-27, 1997。
7. Wu G, Shan X, Li M, Shi S, Zheng Q, Yu L, and Zhao S. A preliminary study on the loss of heterozygosity at 17p13 in gastric and colorectal cancers.  China Natl J New Gastroenteral, 3(3): 160 – 162, 1997.
8. 施少林,余龙,吴国俊,郑其平,范玉新,赵寿元。人类“锌指” 蛋白基因ZNF191 cDNA 的分离与克隆。 自然科学进展, 1997,7:499-502 .
9. 郑其平,余龙,张山,施少林,吴国俊等。 中国人群dystrophin基因结构的初步研究。中国临床神经学杂志。10(5): 262-265, 1997.
张民,余龙,胡培蓉,施少林,吴国俊,赵寿元。一种改良的基因组大片段DNA 探针制备法及其应用。 中华医学遗传学杂志, 14(2):112-114, 1997.
10. 余龙, 宫立群,邓余,施少林,赵寿元。一种用于PCR—SSCP 和STR 分析的银染PAG 胶膜片简易制备法。中华医学遗传学杂志, 13(3): 177-178, 1996.
11. 施少林,余龙,刘孟珉,邓余,张民,龚若沐,赵寿元,谈家桢。从酵母人工染色体的人基因组片段直接分离表达顺序的初步研究。复旦学报(自然科学版),34(4):361-369, 1995.
12. 叶芳耘,支莉,施少林, 何建文, 辛宏, 于洋。淋球菌隐蔽性质粒的克隆和其PCR在临床上的应用。 临床检验杂志,13(2): 71-73, 1995.
13. 陶学训,李芳萍,黄蓓蓓,施少林,匡冰,杨俊。江西省汉族人群近亲婚配研究.  江西中医学院院报, 3(2): 132-133, 1990. 
14. 陶学训,李芳萍,黄蓓蓓,施少林,匡冰,杨俊。 中国江西省89万人群中若干种遗传疾病流行调查.   江西中医学院院报, 3(1): 53-55, 1990.
 
综述及专著章节
1. Ye Y, Song H, Zhang J, Shi S. Understanding the biology and pathogenesis of podocytes by single-cell RNA-seq. Kidney dis. 2018 online. (*通讯作者)
2. Xia H, Bao W, Shi S. Innate Immunity Activity in Glomerular Podocytes. Frontiers in Immunology. 2017 Feb 8;8:122.
3. Shi S, Liu Z. Roles for microRNAs in Podocytopathies. In Podocytopathy, Ed. Liu Z, He JC, Karger Publishing, 2014.
25:456-460,2016. Shi S, Stanley P. Glycosylation in Development. In Glycobiology Ed. Clare Sansom and Ofer Markman. Scion Publishing Ltd. 2007.
4. Shi S, Stanley P. Evolutionary Origins of Notch Signaling in Early Development. Cell Cycle. 5(3):274- 278.  2006. 
5. Shi S, Williams S, Kurniawan H, Lu L, Stanley P. Roles of complex and hybrid N-glycans and O-fucose glycans in oocyte development and function. Adv Exp Med Biol, 2005.
6. 何静, 施少林. DAMPs和肾脏疾病。肾脏病与透析肾移植杂志,23:163-167,2014.
7. 鲍文朵娜, 施少林. Toll样受体9的功能及其致病机制。肾脏病与透析肾移植杂志,25(5                      

... 更多
 
分享至:

学术任务如下:

日期 时间 会场 Session/主题 角色 姓名 单位
2018-09-20 09:40-10:00 第七会场(二层会议室4)

基础研究 session1

单细胞组学技术:现状与进展
讲者

施少林

中国人民解放军东部战区总医院